Journal: Animals : an Open Access Journal from MDPI

Article Title: Effects of Different Ways of Music Stimulation on Exploring, Playing and Aggressive Behavior

doi: 10.3390/ani15182721

Figure Lengend Snippet: The protein expression of emotion-related genes in the hippocampus. ( A ) The protein expression of BDNF, DCX, and EGR1 in hippocampal tissues. ( B – D ) The protein density was quantified using Image J software. BA means baicalin (n = 3). Results are presented as mean ± SE. * p < 0.05, ** p < 0.01, *** p < 0.001. Absence of * indicates no significance.

Article Snippet: EGR1 , 1:1000 , 82 , rabbit , WANLEIBIO, Shenyang, China.

Techniques: Expressing, Software

Journal: Cell & Bioscience

Article Title: KDM6B inhibition enhances chemotherapeutic response in small cell lung cancer via epigenetic regulation of apoptosis and ferroptosis

doi: 10.1186/s13578-025-01496-6

Figure Lengend Snippet: Gskj1 enhances H3K27me3 enrichment at EGR3 and suppresses EGR3/c-FOS signaling to promote apoptosis and ferroptosis. A Volcano plot of differentially expressed genes (DEGs) in H69-AR cells treated with Gskj1 versus DMSO, based on RNA-seq analysis (|log₂FC|> 1, P < 0.05). B KEGG pathway enrichment analysis of DEGs showing involvement in apoptosis and tumor progression. C GSEA analysis indicating significant enrichment of pathways related to apoptotic regulation. D Genome-wide H3K27me3 peak signal profiles from ChIP-seq ( GSE150600 ), showing increased H3K27me3 occupancy at promoter regions and ± 3 kb around transcription start sites following KDM6A/B inhibition. E Heatmap of H3K27me3 signal intensity changes across gene bodies and promoters in the presence or absence of KDM6A/B inhibition. F Venn diagram identifying 13 overlapping genes between downregulated RNA-seq DEGs and genes with increased H3K27me3 peaks, including EGR3 and RBFOX3. G Protein–protein interaction network (STRING database) among overlapping candidates and tumor-related genes, showing co-expression of EGR3 and RBFOX3 with c-FOS (confidence > 0.7), as well as predicted co-expression between KDM6B and c-FOS. H H3K27me3 signal heatmaps for candidate genes (EGR3, RBFOX3, SOX2, MYC, TP53, GATA3) showing increased occupancy, especially at the EGR3 promoter and gene body regions following KDM6A/B inhibition. I Peak signal intensity plots further confirming enhanced H3K27me3 enrichment at EGR3 loci. J Western blot validation of EGR3 and c-FOS downregulation following Gskj1 treatment, accompanied by increased H3K27me3 levels. K Co-immunoprecipitation (Co-IP) analysis demonstrating protein–protein interaction between EGR3 and c-FOS. L Dual-luciferase reporter assay showing EGR3 activates the c-FOS promoter, while mutation abolishes this effect

Article Snippet: For each immunoprecipitation reaction, 500–1000 μg of total protein was incubated overnight at 4 °C with 2–5 μg of anti-EGR3 antibody (Abmart, #PK44695) or anti-c-FOS antibody (Abmart, # T56596 ).

Techniques: RNA Sequencing, Genome Wide, ChIP-sequencing, Inhibition, Expressing, Western Blot, Biomarker Discovery, Immunoprecipitation, Co-Immunoprecipitation Assay, Luciferase, Reporter Assay, Mutagenesis